I have experimented with my media and found this to be the best mix thusfar: 6 Tablespoons potato flakes, 2 tablespoons powdered milk, 1/2 tablespoon sugar, 1/2-1 tablespoon brewers yeast. Use 1/2 cup distilled water, microwave for a minute (till hot or boiling). Mix the dry ingredients in your culturing cup and after pulling the hot water out of the microwave, add the cider vinegar to the water (and food coloring if desired), stir briefly and pour ALL AT ONCE into the culturing cup with the dry ingredients. Immediately stir quickly as it will solidify fast...don't worry if you don't get all the powder mixed in. Replace the lid on the culturing cup while you let the media cool down (so that wild fruit flies won't get in there & lay eggs while your back is turned!) Give it about 15 minutes to cool off, then remove the lid, add a pinch of baking yeast (live, active stuff), add excelsior or folded coffee filters & then add your starter flies.
I have tried several variations of this media and others, and so far this is the one that works the best & produces the fastest. The cider vinegar is not necessary, but may inhibit mold growth and seems to get the little buggers to start laying eggs a lot faster. (I've left it out and it seemed like it took another week or so before they started laying eggs.) You can probably also use white vinegar, some do, but I find the cider stuff works better for me...probably because it has that rotten apple smell.
I have also found that a thick, slightly "dry" mix seems to work better than one that is too soft. When the maggots start hatching out, they "spit" (or something) into the media and make it thinner, so if you make it thin from the get-go, it will be soupy when the maggots start to appear and it will be pouring out on you while you're trying to tap out flies.
If you need a supplier for cheap brewers' yeast, send me a PM...I'm going to be offering it on our website, but I don't have it up there yet!
Good Luck!
-Carey Kurtz-
Green Oasis Reptiles