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Mantid Discussions
Food and Feeding
gonna do a hydei experiment!
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<blockquote data-quote="agent A" data-source="post: 345147" data-attributes="member: 2948"><p>hey all</p><p></p><p>so I have been making my own fruitfly cultures for years</p><p></p><p>first, I dissolve some molasses in warm water and stir in yeast (I let this sit for about 20 minutes)</p><p></p><p>then, I mix applesauce and vinegar together until it's really runny</p><p></p><p>then, I add baby cereal to the applesauce/vinegar mix until it's pretty darn stiff</p><p></p><p>then i add the water/yeast/molasses mix and make it runny once again</p><p></p><p>i pour it into cups, shove a paper towel in, add excelsior, and let it sit for 2-3 days before adding the adult flies, and walla!</p><p></p><p>but it seems that while this is fine for <em>Drosophila melanogaster, </em>it is suboptimal for <em>D. hydei</em></p><p></p><p>so I am going to try some different culture media, including mantisplace media, fruitfly shop media, and repashy media</p><p></p><p>maybe i'll incorporate some of the cornell university recipes too</p><p></p><p>so follow along with me if you want to see how this experiment goes! I will probably make test tubes of each medium so I can place single pairs in it and collect data on development time and fecundity. this will also give me better replication! yay science!</p></blockquote><p></p>
[QUOTE="agent A, post: 345147, member: 2948"] hey all so I have been making my own fruitfly cultures for years first, I dissolve some molasses in warm water and stir in yeast (I let this sit for about 20 minutes) then, I mix applesauce and vinegar together until it's really runny then, I add baby cereal to the applesauce/vinegar mix until it's pretty darn stiff then i add the water/yeast/molasses mix and make it runny once again i pour it into cups, shove a paper towel in, add excelsior, and let it sit for 2-3 days before adding the adult flies, and walla! but it seems that while this is fine for [I]Drosophila melanogaster, [/I]it is suboptimal for [I]D. hydei[/I] so I am going to try some different culture media, including mantisplace media, fruitfly shop media, and repashy media maybe i'll incorporate some of the cornell university recipes too so follow along with me if you want to see how this experiment goes! I will probably make test tubes of each medium so I can place single pairs in it and collect data on development time and fecundity. this will also give me better replication! yay science! [/QUOTE]
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Mantid Discussions
Food and Feeding
gonna do a hydei experiment!
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